Knowledge-enriched characterization of mobile populations. Morphological information and facts from photographs can insert on the richness of flow cytometry facts. As an example, the figure displays an or else typical apoptosis assay utilizing Annexin V and PI, including cell imaging to characterize cells in Just about every inhabitants to reveal morphologically distinct options. These insights couldn't have already been acquired from multiplex staining by yourself.
As samples are acquired around the Attune CytPix Flow Cytometer, the large-pace brightfield camera captures and retailers photos of detected occasions in a price approximately 6,000 photographs per 2nd, according to the flow charge and graphic measurement. For larger overall flexibility, Attune Cytometric Computer software lets you adjust the graphic seize frequency as desired and select a particular gate for picture seize, if expected.
Combine acoustic concentrating technologies with superior-parameter Examination for versatile spectral unmixing and traditional workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing which has a large-speed brightfield digicam for simultaneous higher throughput flow cytometry and higher resolution brightfield imaging.
"An image just isn't very worth a thousand dots, but it really actually helps you to see images. It is excellent to determine photos that correlate with staining. By way of example, AnnexinV-optimistic cells which might be morphologically diverse than nutritious cells, or CD14+ monocytes that are bigger and more textured than CD3+/CD4+/CD14- lymphocytes.
Additionally, it is illuminating to find out the amount of doublets slip by means of common singlet gates, and wonderful to validate the status of unanticipated double-good events in my Assessment."
Optimizing gating system. Even sturdy manual singlet gating is mistake-vulnerable and stays a here subjective decision place in Pretty much all flow cytometry assays. Imaging may be used to verify and regulate gates to include only single cells of curiosity.
Our experts will design and style panels for you personally as often times you'll need—cost-free, no merchandise obtain essential.
Early indications of this contamination had in the beginning been dismissed as particles. Backgating to find new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also enables you to use morphological features to find attention-grabbing subpopulations that could not be clear from flow cytometry knowledge alone.
People can look at celebration photographs in serious-time to confirm sample high quality, optimize protocols, and record the morphology of each and every mobile inhabitants recognized for Investigation. Imaging capture also Added benefits from acoustic concentrating, which helps you to posture the cells to make sure that a sharp, centered impression is obtained.
AI-driven automated graphic Evaluation Using the Attune Cytometric Application translates occasion functions into distinct morphology parameters that may be coupled with common fluorescence and scatter parameters to reveal really reproducible populace changes and distinctive subsets.
Application Notice: Automatic image Examination lowers user-to-user variability in flow cytometry gating strategies
Leverage the strength of advanced technological innovation and innovation to speed up your discoveries. Have confidence in within the Attune Flow Cytometer household to provide the reliability, speed, and accuracy that set your operate apart.
Adaptable payment program from Agilent Economical Options let you get the vital instrumentation you'll need for speedy challenge, or to expand your lab.
Gating on these extended parameters permits you to promptly and properly discover populations of desire and make sure gating technique with little or no guide evaluation. The Attune software features usage of state-of-the-art equipment Discovering Evaluation applications in an expertly built person interface, which makes it extremely convenient to use.
The Attune Xenith cytometer is a substantial-parameter spectral analyzer, furnishing the two spectral unmixing and traditional flow capabilities for thorough and adaptable mobile Examination.
The Attune CytPix Flow Cytometer allows consumers to visualise samples in the course of acquisition, confirm the precision of gating strategies, and help outcomes with responsible documentation for publication applications.
What gave the impression to be a homogeneous population is actually additional varied—an insight that ought to be regarded as when interpreting final results.